Journal: Frontiers in Medicine

Article Title: Investigating antibacterial and anti-inflammatory properties of synthetic curcuminoids

doi: 10.3389/fmed.2024.1478122

Figure Lengend Snippet: Antimicrobial activity of Py-cPen and V-cPen in vitro . VCA was used to analyze the antibacterial effects of Py-cPen (5, 10, and 30 μM) and V-cPen (1, 3, 10, and μM). LL37 (1 μM) was used as a positive control, and untreated (using TRIS) bacteria were used as a negative control. The concentration of bacteria ( E. coli , P. aeruginosa , and S. aureus ) was 2 × 10 6 CFU/mL, and each sample was treated for 2 h. Data are presented as the mean ± SEM of four independent experiments ( n = 4). Statistical analysis was performed using a one-way ANOVA with Dunnett’s multiple comparison tests. Results are reported as ns = not significant, * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

Article Snippet: Cathelicidin antimicrobial peptide LL37 was purchased from Innovagen (United States) ( , ).

Techniques: Activity Assay, In Vitro, Positive Control, Bacteria, Negative Control, Concentration Assay, Comparison

Journal: Frontiers in Medicine

Article Title: Investigating antibacterial and anti-inflammatory properties of synthetic curcuminoids

doi: 10.3389/fmed.2024.1478122

Figure Lengend Snippet: Visualization of E. coli , P. aeruginosa , and S. aureus viability. LIVE/DEAD Viability Assay of Gram-negative and Gram-positive bacteria stimulated with Py-cPen (10 μM) or V-cPen (3 μM), 10 mM Tris buffer at pH 7.4 as a negative control and LL37 (1 μM) as a positive control. Representative images for each independent experiment are presented ( n = 4). Live bacteria were stained with green SYTO 9 nucleic acid fluorescent dye, and dead bacteria were stained with red propidium iodine dye.

Article Snippet: Cathelicidin antimicrobial peptide LL37 was purchased from Innovagen (United States) ( , ).

Techniques: Viability Assay, Bacteria, Negative Control, Positive Control, Staining

Journal: Frontiers in Medicine

Article Title: Investigating antibacterial and anti-inflammatory properties of synthetic curcuminoids

doi: 10.3389/fmed.2024.1478122

Figure Lengend Snippet: Inhibition of NF-κB activation using Py-cPen and V-cPen. (A) THP-1XBlue-CD14 cells were treated with Py-cPen (10–30 μM). In the case of Py-cPen, there is a significant reduction in the activation of NF-κB. (B) THP-1XBlue-CD14 cells were also treated with V-cPen (3–10 μM). V-cPen yielded the enhancement of NF-κB activation. LL37 (1 μM) was used as a positive control. The MTT viability assay was used to analyze the toxic effects of Py-cPen and V-cPen on THP-1XBlue-CD14 cells. Data are presented as the mean ± SEM of four independent experiments ( n = 4). Statistical analysis was performed using a one-way ANOVA with Dunnett’s multiple comparison tests. Results are reported as ns = not significant and **** p < 0.0001.

Article Snippet: Cathelicidin antimicrobial peptide LL37 was purchased from Innovagen (United States) ( , ).

Techniques: Inhibition, Activation Assay, Positive Control, MTT Viability Assay, Comparison